Description
The GenNext Flash Oxidation (Fox®) System is a laser-free benchtop platform designed for Hydroxyl Radical Protein Footprinting (HRPF) to study protein higher-order structure (HOS). It provides a safer and more automated alternative to traditional laser-based methods like Fast Photochemical Oxidation of Proteins (FPOP). Core Technology and Workflow The system utilizes a proprietary high-pressure plasma flash lamp rather than a Class IV laser to generate hydroxyl radicals from hydrogen peroxide (H2O2cap H sub 2 cap O sub 2𝐻2𝑂2). The GenNext Flash Oxidation (Fox®) System is a modular, semi-automated entry-level system suitable for researchers who do not require high-throughput processing Core Technology and Workflow Labeling: Hydroxyl radicals irreversibly modify solvent-exposed amino acid side chains during a 10 📷μmu𝜇s flash. Dosimetry: An in-line Radical Dosimeter measures radical dose in real-time, automatically adjusting for background scavenging to ensure high reproducibility. Collection: A programmable Product Collector automatically quenches and harvests properly oxidized samples while diverting waste. Analysis: Data is processed via FoxWare® Software , which automates the identification and quantification of oxidative modifications at the peptide and residue level. Primary Applications Epitope/Paratope Mapping: Identifies binding interfaces for monoclonal antibodies (mAbs) with precision matching X-ray crystallography. Aggregation Analysis: Detects intermolecular interactions and identifies aggregation-prone regions in protein formulations. Biosimilar Comparison: Provides high-resolution structural comparisons between reference biologics and biosimilar candidates. AI Model Validation: Rapidly adjudicates AI-generated structural predictions (e.g., AlphaFold) in days rather than months.